I-Carbon esetshenzisiwe: Uhlobo lwe-adsorbent engeyona eye-polar esetshenziswe ngaphezulu. Ngokuvamile, kudinga ukugezwa nge-dilute hydrochloric acid, kulandelwe yi-ethanol, bese kugezwa ngamanzi. Ngemuva kokumiswa ngo-80 ℃, ingasetshenziselwa ikholomu ye-chromatography. I-Granur Carbon ecushiwe yindlela engcono kakhulu yekholomu i-chromatography. Uma kungumpuphu omuhle wekhabhoni eyenziwe nge-carbon, kuyadingeka ukwengeza inani elifanele le-diatomite njengosizo lokuhlunga, ukuze ugweme ukugeleza okuhamba kancane.
I-Carbon ecushiwe i-adsorbent engeyona polar. Ama-adsorption alo aphambene ne-silica gel no-alumina. Inobudlelwano obuqinile bezinto ezingezona ze-polar. Inamandla aqine kakhulu ama-adsorption esikhangini esinamanzi futhi ibuthakathaka ku-organic solvent. Ngakho-ke, amandla okuxazulula amanzi abuthakathaka futhi i-organic solvent inamandla. Lapho into e-adsorbed ifakwa kwikhabhoni eyenziwe yasebenza, ubumbano lwe-solvent luyancipha, futhi umthamo we-advocate we-advocate ye-carbon evuliwe, futhi amandla okusa kwe-eluent ayathuthukiswa. Izakhi ezingena emanzini, njengama-amino acid, ushukela kanye ne-glycosides, zazodwa.